Roche’s new detection kits have more translucent dyes which are suited for mRNA ISH co-localization studies

Swiss healthcare company Roche has recently announced that it has expanded its collaboration with Bio-Techne by providing drug discovery researchers in the U.S. with novel chromogenic detection kits for ISH (in situ hybridization) tissue analysis.

Apparently, the novel Roche kits are comprised of the DISCOVERY mRNA Purple HRP and Teal HRP detection kits. Both the teal and purple detection kits have more translucent dyes which are suited for mRNA ISH co-localization studies and feature the ability to perform ISH and immunohistochemistry (IHC) for co-detecting protein-RNA on same slides.

Citing reliable sources, designs of both these kits are aimed for using on Roche’s staining platform DISCOVERY ULTRA along with the enhanced signal-to-noise ratio and robustness of its newly released ACD RNAscope VS Universal HRP Reagent Kit.

Sources informed that mRNA Duplex AMP kit from Roche now has an extended application that allows dual mRNA ISH detection by using the latest mRNA Teal detection kit with the already available mRNA RED detection kit.

The red & teal combination offers a different visual contrast and the teal dye’s translucency enables the studying of mRNA ISH marks co-expressions, where the subsequent red and teal dyes overlap to create an indigo color. This new application is proposed for use with the newly launched RNAscope VS Duplex Reagent Kit that allows real-time in situ detection of two mRNA species.

Seemingly, the dual mRNA ISH application and new detection kits allow researchers to raise their multiplexing possibilities, while optimizing staining conditions for challenging probes and antibodies, conserving tissue samples, saving time and easily running complex assays.

Automating IHC and ISH on the DISCOVERY ULTRA platform allows scientists to create reproducible, standardized results and remove more than 90% of manual processes so that they can devote more time focusing on their research part, sources commented.

Source Credit: https://www.biospace.com/article/releases/roche-expands-partnership-with-bio-techne-to-offer-drug-discovery-researchers-new-chromogenic-detection-options-for-mrna-ish-tissue-analysis/